U-87 MG-CAS9 cell line

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the U-87 MG cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a U-87 MG-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
U-87 MG-CAS9	Cas9	16.77473450	1.18480969
U-87 MG-CAS9	β-actin	15.58992481	1.18480969
U-87 MG	Cas9	36.17204666	20.33946323
U-87 MG	β-actin	15.83258343	20.33946323

Cutting Efficiency Validation

U-87 MG-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the U-87 MG-CAS9 stable cell pool which is electroporated by HMOX1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U-87 MG			STR Info (Cell bank) Cell Line:U-87MG
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	8	11		8	11
D7S820	8	9		8	9
D16S539	12	12		12	12
VWA	15	17		15	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	10	11		10	11
D12S391	18	21
FGA	18	24
D2S1338	20	23
D21S11	28	32.2
D18S51	13	13
D8S1179	10	11
D3S1358	16	17
D6S1043	11	18
PENTAE	7	14
D19S433	15	15.2
PENTAD	9	14
D1S1656	15	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

U-87 MG-CAS9

Catalog#

YC-C028-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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U-87 MG-CAS9 cell line

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Product Advantage

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IDH1 Knockout cell line (U-87 MG)
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SLC7A11 Knockout cell line (U-87 MG)
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HMOX1 Knockout cell line (U-87 MG)
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